Alkynyl-functionalized graphene oxide (GO) is a gorgeous nanomaterial for a lot of purposes. Controlling the functionalization of GO is crucial for the design of GO derivatives with novel properties. Right here, we reported a brand new technique to conveniently management the diploma of alkynyl-functionalization of GO by manipulating the response temperature. The alkynyl-functionalized GO was characterised by way of Zeta potential, Fourier rework infrared spectroscopy, Raman spectroscopy, X-ray diffraction, and Thermogravimetric evaluation, confirming the covalent modification of the GO floor with propargylamine. The outcomes confirmed that GO was efficiently functionalized by propargylamine. And the diploma of alkynyl-functionalization of GO will increase with the rise in response temperature. The results of the temperature on the mechanism of alkynyl-functionalized GO have been additionally revealed. This methodology might be exploited to organize useful GO with potential purposes in lots of fields.